Low hepatic manganese concentrations in patients with hepatic steatosis – A cohort study of copper, iron and manganese in liver biopsies

Hepatic steatosis is the most common histopathological finding on liver biopsy, with prevalent etiology being NAFLD. The pathogenesis of hepatic and NAFLD multifactorial, however, studies importance manganese in are limited. We aimed to study content, other trace elements, relation patients chronic diseases different etiology, mainly Patients chronically elevated function tests underwent a diagnostic work-up, including routine blood two biopsies. One biopsies was sent for evaluation, ultra-trace elemental determinations. Steatosis graded using conventional methodology, fat content also quantitated biopsy samples by measuring steatotic area section stereological point counting (SPC). Ultra-trace analysis utilized determining manganese, iron, copper inductively coupled plasma sector field mass spectrometry (ICP-SFMS). 76 were included study. concentrations lower than without (3.8 ± 1.1 vs. 6.4 1.8, P < 0.001). Similar results seen levels steatosis. found strong inverse correlation between grade (?=-0.743, Also, low independently predicted presence (aOR 0.07 [95%CI: 0.01?0.63]). NAFLD, or CLD concomitant steatosis, showed increasing